Editing Revisiting puzzle/75: Antifreeze Protein

[[File:Irc 190318 1433750142 S1 BitSpawn.png|thumb|400px|BitSpawn's AFP from [[Puzzle 1095|puzzle 1095]].]]
[[Revisiting_puzzle/Antifreeze Protein|Antifreeze Protein]] is a [[Revisiting puzzle|revisiting puzzle]], originally presented in 2008 as [https://fold.it/portal/node/395222 Puzzle 75: Antifreeze Protein].

The protein in this puzzle is an antifreeze protein (AFP), which cold water fishes use to keep from freezing. This protein is from a type of fish known as an eelpout, specifically an [[wikipedia:Ocean_pout|ocean pout]], ''Zoarces americanus''.

The same protein also appears in the [[Revisiting_puzzle/Ice_Binding|Ice Binding]] series of revisiting puzzles.

The antifreeze AFPs are not be confused with [[wikipedia:Alpha-fetoprotein|alpha-fetoprotein]], also known as AFP.

The puzzle has 66 segments with the sequence:

<pre> 
anqasvvanqlipintaltlvmmrsevvtpvgipaediprlvsmqvnravplgttlmpdmvrgyaa
</pre>

This sequence is found in the following [[PDB|Protein Data Bank]] entry:
*[https://www.rcsb.org/structure/1b7i 1B7I] (chain A, offset 0)

There are also other PDB entries which match 65 out of 66 segments:
*[https://www.rcsb.org/structure/1ame 1AME] (chain A, offset 1)
*[https://www.rcsb.org/structure/5c7r 5C7R] (chains A and B, offset 370)

In 1AME and 5C7R, the mismatch is at segment 62 of the Foldit protein. In both cases, the arginine (R) at segment 62 is lysine (K) in the PDB protein. 

This puzzle does not have [[Disulfide Bridge|disulfide bridges]].

This puzzle does not have any [[Ligand|ligands]].

This puzzle has a single [[Chain|chain]].

See players' solutions from previous visits to this puzzle:
*[[Puzzle 777]]
*[[Puzzle 1095]]
*[[Puzzle 1562]]
*[[Puzzle 1750]]
*[[Puzzle 2290]]
Complete series of revisits with top scores: 
{| class="wikitable"
|-
!puzzle
!closed
!top solo score
!top solo
!top evo score
!top evo
!top group score
!top group
!comments
|-
|[https://fold.it/portal/node/395222 75: Antifreeze Protein]
|08/28/08
|9,567
|Franco1947
|9,572
|gla
|9,572
|Void Crushers
|
|-
|[https://fold.it/portal/node/995907 777: Revisiting Puzzle 75: Antifreeze Protein]
|09/18/13
|9,640
|Wilm
|9,640
|gloverd
|9,640
|Go Science
|
|-
|[https://fold.it/portal/node/2000785 1095: Revisiting Puzzle 75: Antifreeze Protein]
|06/08/15
|8,596
|BitSpawn
|8,585
|mimi
|8,596
|Contenders
|
|-
|[https://fold.it/portal/node/2002963 1298: Revisiting Puzzle 75: Antifreeze Protein]
|10/26/16
|9,001
|markm457
|9,009
|Galaxie
|9,009
|Anthropic Dreams
|
|-
|[https://fold.it/portal/node/2002992 1300: Revisiting Puzzle 75 with Density: Antifreeze Protein]
|11/02/16
|12,709
|markm457
|12,710
|gauchomurphy
|12,710
|Anthropic Dreams
|
|-
|[https://fold.it/portal/node/2005581 1562: Revisiting Puzzle 75: Antifreeze Protein]
|08/22/18
|10,181
|matosfran
|10,181
|matosfran
|10,181
|Beta Folders
|hat trick matosfran
|-
|[https://fold.it/portal/node/2008228 1750: Revisiting Puzzle 75: Antifreeze Protein]
|10/30/19
|10,176
|Bruno Kestemont
|10,170
|Bruno Kestemont
|10,173
|Go Science
|hat trick Bruno K.
|-
|[https://fold.it/portal/node/2011957 2030: Revisiting Puzzle 75: Antifreeze Protein]
|08/19/21
|10,187
|grogar7
|10,195
|Galaxie
|10,195
|Anthropic Dreams
|
|-
|[https://fold.it/puzzles/2013607 2290: Revisiting Puzzle 75: Antifreeze Protein]
|04/26/23
|10,161
|Wanderer09
|10,153
|Galaxie
|10,153
|Anthropic Dreams
|
|}
[[Category:Revisiting Puzzle]]
[[Category:Revisiting Puzzle 75: Antifreeze Protein]]